Heavy Metals and Antibiotic co-Resistance in Bacterial Isolates of Industrial Effluents

نوع مقاله : مقاله پژوهشی

نویسنده

Assoc. Prof., Dept. of Basic Medical Sciences, Isfahan (Khorasgan)Branch, Islamic Azad University, Isfahan, Iran

چکیده

Heavy metal and antibiotic co-resistance is a global issue. The goal of this research was to explore the heavy metal also antibiotic resistance patterns of effluent bacterial isolates. Heavy metal resistant bacteria were isolated from effluents and their Minimum Inhibitory Concentration (MIC) was determined. The Multi-Metal resistance (MMR) pattern and antibiotic resistance trait of isolates were defined. The MIC of Cu2+, Pb2+, Cd2+ and Zn2+ was 4, 8, 12 and 24 mM/L, respectively. Most of the isolates indicated the Cd2+, Pb2+ and Zn2+ resistance and high resistance to the most tested antibiotics. The 16S rDNA gene sequences of resistant isolates were handed over to NCBI-GenBank as Staphylococcus sp. ATHA2(JX120151) and Klebsiella oxytoca ATHA1(JQ928574). Between metal tolerances, heavy metal concentration also antibiotic resistance in bacteria existed a correlation. Thus, we’d better not only be alert of antibiotics misapplication, but also responsive of over discharge of effluent containing heavy metals to the environment.

کلیدواژه‌ها


عنوان مقاله [English]

Heavy Metals and Antibiotic co-Resistance in Bacterial Isolates of Industrial Effluents

نویسنده [English]

  • Arezoo Tahmourespour
Assoc. Prof., Dept. of Basic Medical Sciences, Isfahan (Khorasgan)Branch, Islamic Azad University, Isfahan, Iran
چکیده [English]

Heavy metal and antibiotic co-resistance is a global issue. The goal of this research was to explore the heavy metal also antibiotic resistance patterns of effluent bacterial isolates. Heavy metal resistant bacteria were isolated from effluents and their Minimum Inhibitory Concentration (MIC) was determined. The Multi-Metal resistance (MMR) pattern and antibiotic resistance trait of isolates were defined. The MIC of Cu2+, Pb2+, Cd2+ and Zn2+ was 4, 8, 12 and 24 mM/L, respectively. Most of the isolates indicated the Cd2+, Pb2+ and Zn2+ resistance and high resistance to the most tested antibiotics. The 16S rDNA gene sequences of resistant isolates were handed over to NCBI-GenBank as Staphylococcus sp. ATHA2(JX120151) and Klebsiella oxytoca ATHA1(JQ928574). Between metal tolerances, heavy metal concentration also antibiotic resistance in bacteria existed a correlation. Thus, we’d better not only be alert of antibiotics misapplication, but also responsive of over discharge of effluent containing heavy metals to the environment.

کلیدواژه‌ها [English]

  • Antibiotic
  • Bacteria
  • Co-Resistance
  • heavy metals
  • Industrial Effluent
Abidin, Z. A. Z. & Chowdhury, A. J. K. 2018. Heavy metals and antibiotic resistance bacteria in marine sediment of Pahang coastal water. Journal Clean WAS, 2, 20-22.
Alboghobeish, H., Tahmourespour, A. & Doudi, M. 2014. The study of Nickel Resistant Bacteria (NiRB) isolated from wastewaters polluted with different industrial sources. Journal of Environmental Health Science and Engineering, 12, 1-7.
Ansari, M. I. & Malik, A. 2007. Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater. Bioresource Technology, 98, 3149-3153.
Azam, M., Jan, A. T., Kumar, A., Siddiqui, K., Mondal, A. H. & Haq, Q. M. 2018. Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna. Brazilian Journal of Microbiology, 49, 471-480.
Chen, B. Y., Wu, C. H. & Chang, J. S. 2006. An assessment of the toxicity of metals to Pseudomonas aeruginosa PU21 (Rip64). Bioresource Technology, 97, 1880-1886.
Esa, S. K., Haque, A. A. M. & Murshed, M. 2013. Performance of sewage oxidation pond in USM engineering campus. Caspian Journal of Applied Science Research, 2, 219-225.
Filali, B., Taoufik, J., Zeroual, Y., Dzairi, F., Talbi, M. & Blaghen, M. 2000. Wastewater bacterial isolates resistant to heavy metals and antibiotics. Current Microbiology, 41, 151-156.
Haroun, A., Kamaluddeen, K., Alhaji, I., Magaji, Y. & Oaikhena, E. 2017. Evaluation of heavy metal tolerance level (MIC) and bioremediation potentials of Pseudomonas aeruginosa isolated from Makera-Kakuri industrial drain in Kaduna, Nigeria. European Journal of Experimental Biology, 7(5), 28.
Harris, S. J., Cormican, M. & Cummins, E. 2012. Antimicrobial residues and antimicrobial-resistant bacteria: impact on the microbial environment and risk to human health-a review. Human and Ecological Risk Assessment: an International Journal, 18, 767-809.
Hasan, M. M., Hosain, S., Poddar, P., Chowdhury, A. A., Katengeza, E. W. & Roy, U. K. 2019. Heavy metal toxicity from the leather industry in Bangladesh: a case study of human exposure in Dhaka industrial area. Environmental Monitoring and Assessment, 191, 530.
Kacar, A. & Kocyigit, A. 2013. Characterization of heavy metal and antibiotic resistant bacteria isolated from Aliaga Ship Dismantling Zone, Eastern Aegean Sea, Turkey. International Journal of Environmental Research, 7, 895-902.
Keramati, P., Hoodaji, M. & Tahmourespour, A. 2011. Multi-metal resistance study of bacteria highly resistant to mercury isolated from dental clinic effluent. African Journal of Microbiology Research, 5, 831-837.
Khan, G. A., Berglund, B., Khan, K. M., Lindgren, P. E. & Fick, J. 2013. Occurrence and abundance of antibiotics and resistance genes in rivers, canal and near drug formulation facilities–a study in Pakistan. PloS One, 8, e62712.
Knapp, C. W., Mccluskey, S. M., Singh, B. K., Campbell, C. D., Hudson, G. & Graham, D. W. 2011. Antibiotic resistance gene abundances correlate with metal and geochemical conditions in archived Scottish soils. PloS One, 6, e27300.
Lawe-Davies, O. & Bennett, S. 2017. WHO-list of bacteria for which new antibiotics are urgently needed. WHO Department of Communications.
Li, L. G., Xia, Y. & Zhang, T. 2017. Co-occurrence of antibiotic and metal resistance genes revealed in complete genome collection. The ISME Journal, 11, 651-662.
Marti, E., Variatza, E. and Balcazar, J. 2014. The role of aquatic ecosystems as reservoirs of antibiotic resistance. Trends in Microbiology, 22, 36-41.
Mojiri, A., Vakili, M., Farraji, H. & Aziz, S. Q. 2019. Combined ozone oxidation process and adsorption methods for the removal of acetaminophen and amoxicillin from aqueous solution; kinetic and optimisation. Environmental Technology and Innovation, 15, 100404.
Mustapha, M. U. & Halimoon, N. 2015. Screening and isolation of heavy metal tolerant bacteria in industrial effluent. Procedia Environmental Sciences, 30, 33-37.
Nwuche, C. & Ugoji, E. 2008. Effects of heavy metal pollution on the soil microbial activity. International Journal of Environmental Science and Technology, 5, 409-414.
Pal, C., Asiani, K., Arya, S., Rensing, C., Stekel, D. J., Larsson, D. J., et al. 2017. Metal resistance and its association with antibiotic resistance. Advances in Microbial Physiology, 70, 261-313.
Prasanth, S. & Mahesh, V. 2016. Assessment of multi-metal resistant bacteria from Periyar river, Southern India. International Journal of Advances in Scientific Research, 2, 23-26.
Ray, B. & Lamsam, A. 2006. Treatment and management of wastewater from an acrylic fiber industry. GMSARN International Confrence on Sustainable Development: Issues and Prospects for GMS. Bangkok, Thailand.
Rice, E. W., Baird, R. B., Eaton, A. D. & Clesceri, L. S. 2012. Standard methods for the examination of water and wastewater, American Public Health Association, Washington, DC., USA.
Sair, A. T. & Khan, Z. A. 2018. Prevalence of antibiotic and heavy metal resistance in Gram negative bacteria isolated from rivers in northern Pakistan. Water and Environment Journal, 32, 51-57.
Sinegani, A. a. S. & Younessi, N. 2017. Antibiotic resistance of bacteria isolated from heavy metal-polluted soils with different land uses. Journal of Global Antimicrobial Resistance, 10, 247-255.
Tahrani, L., Soufi, L., Mehri, I., Najjari, A., Hassan, A., Van Loco, J., et al. 2015. Isolation and characterization of antibiotic-resistant bacteria from pharmaceutical industrial wastewaters. Microbial Pathogenesis, 89, 54-61.
Tamás, M. J. & Martinoia, E. 2006. Molecular biology of metal homeostasis and detoxification, Springer, Zurich, Switzerland.
Utgikar, V. P., Chaudhary, N., Koeniger, A., Tabak, H. H., Haines, J. R. & Govind, R. 2004. Toxicity of metals and metal mixtures: analysis of concentration and time dependence for zinc and copper. Water Research, 38, 3651-3658.
Verma, S. & Kuila, A. 2019. Bioremediation of heavy metals by microbial process. Environmental Technology and Innovation, 14, 100369.
Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., et al. 2011. Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes. Springer Science and Business Media, New Delhi, India.
World Health Organization, 2015. Antimicrobial Resistance Fact sheet N 194. WHO Publications.
Xiong, J., Zhao, T., Cheng, H., Li, S., Wang, S. & Chen, G. 2019. The assessment on the heavy metal pollution and health risks in the Liujiang river under the Xijiang river region. Desalination and Water Treatment, 149, 315-322.
Yana, F. & Niua, Z. 2019. Evaluation model of major heavy metals pollution factors in coastal waters and sediments. Desalination and Water Treatment, 149, 335-340.
Zheng, T., Zhang, T., Wang, Q., Tian, Y., Shi, Z., Smale, N., et al. 2015. Advanced treatment of acrylic fiber manufacturing wastewater with a combined microbubble-ozonation/ultraviolet irradiation process. RSC Advances, 5, 77601-77609.