Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil

Document Type : Research Paper


1 M.Sc. and Academic Member of Chemistry Eng. Dept., Azad University, Branch of Shahreza

2 M.Sc. and Academic Member of Civil and Environmental Eng., Jami Institute of Tech., Delijan

3 Assoc. Prof. of Environmental Health Eng., Faculty of Public Health, Ahwaz University of Medical Sciences, Ahwaz

4 Assoc. Prof. of Chemical Eng., Dept. of Eng., Isfahan University, Isfahan

5 Assist. Prof. of Chemical Eng., Dept. of Eng., Isfahan University, Isfahan


Contamination of soil by crude oil can pose serious problems to ecosystems. Soil washing by solutions containing biosurfactants is one of the most efficient methods for the remediation of contaminated soil by crude oil because it removes not only the crude oil but also heavy metals. In this study, five soil samples were taken from fields exposed to oil compounds over the years in order to produce biosurfactants from microorganisms that were capable of degrading oil compounds. Sixteen such microorganisms were isolated. After cultivation, their emulsification strength was examined using E24 test. From among the experimental microorganisms, a gram-negative and rod-shape microorganism called A-12 showed the greatest value of the E24 test index (36%). For each liter of the culture medium containing 365 mg of microorganisms, 3 gr of the biosurfactant compound was produced and separated as dried powder. The purified biosurfactant was used in the soil washing process. Also, the insulated microorganisms were capable of degrading crude oil floating on wastewaters.


1- Dibble, J. T., and Bartha, R. (1979). “Effect of environmental parameters on the biodegradation of oil sludge.” Applied and Environmental Microbiology, 37(4), 729-739.
2- William, C., Anderson W.C., and Dee, P. E. (1993). Innovative site remediation technology: Soil washing/soil flushing, EPA, USA.
3- Anderson, R., Rasor, E., and Van Ryn, F. (1999). “Particle size separation via soil washing to obtain volume reduction.” J. of Hazard Malter, 66 (1-2), 89-98.
آب و فاضلاب                                                                                                                                                                      شماره 3 سال 1389
4- Tobia, R.J., Camacho, J.A.M., Augustin, P. Griffiths, R. A., and Frederick, R. M. (1994). “Surfactant treatment of crude oil contaminated soils.” J. of Hazard Mater, 38 (1), 145-161
5- Khodadoust, A. P., Lei, L., Antia, J. E., Bagchi, R., suidan, M. T., and Tabak, H. H. (2005). “Adsorption of PAHs in aged harbor sediments.” ASCE J. of Environmental Egn., 131 (3), 403-409.
6- Mann, M. J. (1999). “Full-scale and pilot- scale soil washing.” J. Hazard. Mater, 66 (1–2), 119-136.
7- Kosaric, N. (1987). Biosurfactants and biotechnology, Marcel Dekker,New York.
8- Hunter, R.J. (1993). Introduction to modern colloid science, Oxford Science Publications,UK.
9- Urum, K., Pekdemir T., and Copur, M. (2004). “Surfactants treatment of crude oil contaminated soils.” J. of Colloid and Interface Science, 276, 456-464.
10- Francy, D. S., Thomas, J. M., Raymond, R. L., and Ward, C. H. (1991). “Emulsification of hydro carbons by surface bacteria.” J. Ind. Microbiol, 8, 237–246.
11- Van Dyke, M. I., Couture, P., Brauer, M., Lee, H., and Trevors, J. T. (1993). “Pseudomonas aeruginosa UG2 rhamnolipid biosurfactants: Structural characterization and their use in removing hydro phobic compounds from soil.” J. Microbiol., 39, 1071-1078.
12- Hamme, J. D., and ward, O. P. (1999). “Influence of chemical surfactants on the biodegradation of crude oil by a mixed- bacterial culture.” Can J. of Micorbiol., 45, 130-137.
13- Maier, R. M., and Sorberon-Ghavaz, G. (2000). “Pseudomona aeruginosa rhamnolipidisL biosynt hesis and potential environmental applications.” Appl. Microbial. Biotech., 54, 625-633
14- Makkar, R. S., and Cameotra, S. S. (1998). “Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis.” J. of Indus. Microb. and Biotech., 20, 48-52.
15- Talaie, A. R. (2008). “Parametric study of petroleum compounds biodegradation using microorganisms,” M.Sc. Thesis, Scince and Research Azad University Branch-Ahvaz.
16- Talaie, A. R., Talaie, M., and Jafaarzadeh, N. (2009) “Optimization of biodegradation of wastewater contaminated floating diesel fuel by Taguchy method.” J. Water and Wastewater, 71, 57-68.
17- Talaie, A. R., Jafaarzadeh, N., Talaie, M., and Beheshti, M. (2009). “Optimization of floating crude oil biodegradation by isolated microorganisms via experimental design method.” J. of Koomesh, 11 (1), 41-54.
18- Qingxin, Li, Kang, C., and Zhang, C. (2005). “Wastewater produced from an oilfield and continuous treatment with and oil-degrading bacterium.” Process Biochemistry, 40, 873-877.
19- Choi, H. D., Hori, K., Tnji, Y., and Unno H. (1974). “Microbial degradation kinetics of solid alkane dissolved in nondegradable oil phase.” J. of Biochemical Engineering, 3, 71-78.
20- Liang, Z. G., Yue-ting, W. U., Xin-ping Q., and Qin, M. (2005). “Biodegradation of crude oil by pseudomonas aeruginosa in the presence of rhamnolipids.” J. of Zhejiang University Science, 8, 725-730.
21- Mulkins-Philips, G. J., and Stewart J. E. (1974). “Effect of four dispersants on biodegradation and growth of bacteria on crude oil.” Applied Microbiology, 28(4), 547-552.