Carbon and Nutrients Removal from Industrial Wastewater Using Aerobic Granular Sludge

Document Type : Research Paper


1 Assist. Prof., Dept. of Applied Chemistry, Faculty of Gas and Petroleum, Yasouj University, Gachsaran, Iran

2 Undergraduate Student, Dept. of Applied Chemistry, Faculty of Gas and Petroleum, Yasouj University, Gachsaran, Iran


Considering the increasing rigor of environmental laws, the removal of carbon and nutrients from wastewater is a key aspect of research, and the simultaneous elimination of carbon and nutrients in a bioreactor has a significant impact on reducing reactor volume and energy consumption. The objective of this study was evaluating the performance of an aerobic sequencing batch reactor (SBR) with granulated sludge removing carbon and nutrient (N & P) from an industrial wastewater. Aerobic granules were obtained in a SBR and in the next step, the experiments were designed by a central composite design (CCD) with five levels of biomass concentration (2000-7000 mg/l) and aeration time (6-24 h).  Eight dependent parameters as the process responses were measured and calculated. The results showed that the maximum value of total COD (TCOD) removal was obtained to be 69.07% at mixed liquor volatile suspended solid (MLVSS) concentration of 5600 mg/L and the highest value of the aeration time (24 h). In addition, the low TN removal (47.5%) directed the study to reduce the oxygen level from 7 to 3 mg/L. A reduction in dissolved oxygen (DO) in extended aeration mode led to an increase in TN removal and a decrease in TCOD, nbCOD, and BOD removal. Overall, granular sludge showed an acceptable performance in terms of carbon removal, however, intermittent aeration could improve nutrients removal from wastewaters.


Ab Halim, M. H., Anuar, A. N., Chelliapan, S., Wahab, N. A., Basri, H. F., Ujang, Z., et al. 2019. Development of aerobic granules in sequencing batch reactor system for treating high temperature domestic wastewater. Jurnal Teknologi, 81. (Science and Engineering), 81(3), 57-66.
Arrojo, B., Mosquera-Corral, A., Garrido, J. M. & Méndez, R. 2004. Aerobic granulation with industrial wastewater in sequencing batch reactors. Water Research, 38, 3389-3399.
Asadi, A., Zinatizadeh, A. & Sumathi, S. 2012. Simultaneous removal of carbon and nutrients from an industrial estate wastewater in a single up-flow aerobic/anoxic sludge bed (UAASB) bioreactor. Water Research, 46, 4587-4598.
Asadi, A., Zinatizadeh, A. A. & Van Loosdrecht, M. 2016. A novel continuous feed and intermittent discharge airlift bioreactor (CFIDAB) for enhanced simultaneous removal of carbon and nutrients from soft drink industrial wastewater. Chemical Engineering Journal, 292, 13-27.
Bathe, S., De Kreuk, M. K., Mcswain, B. & Schwarzenbeck, N. 2005. Aerobic granular sludge, IWA Publishing.
De Kreuk, M. K., Heijnen, J. & Van Loosdrecht, M. 2005. Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge. Biotechnology and Bioengineering, 90, 761-769.
De Kreuk, M. K. & Van Loosdrecht, M. C. 2006. Formation of aerobic granules with domestic sewage. Journal of Environmental Engineering, 132, 694-697.
Fang, F., Liu, X. W., Xu, J., Yu, H. Q. & Li, Y. M. 2009. Formation of aerobic granules and their PHB production at various substrate and ammonium concentrations. Bioresource Technology, 100, 59-63.
Gao, D., Liu, L., Liang, H. & Wu, W. M. 2011. Aerobic granular sludge: characterization, mechanism of granulation and application to wastewater treatment. Critical Reviews in Biotechnology, 31, 137-152.
Guo, H., Chen, J., Li, Y., Feng, T. & Zhang, S. 2013. Nitrogen and phosphorus removal in an airlift intermittent circulation membrane bioreactor. Journal of Environmental Sciences, 25, S146-S150.
Hailei, W., Guangli, Y., Guosheng, L. & Feng, P. 2006. A new way to cultivate aerobic granules in the process of papermaking wastewater treatment. Biochemical Engineering Journal, 28, 99-103.
Hamza, R. A., Iorhemen, O. T., Zaghloul, M. S. & Tay, J. H. 2018. Rapid formation and characterization of aerobic granules in pilot-scale sequential batch reactor for high-strength organic wastewater treatment. Journal of Water Process Engineering, 22, 27-33.
He, Q., Song, Q., Zhang, S., Zhang, W. & Wang, H. 2018. Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sequencing batch reactor with mixed carbon sources: reactor performance, extracellular polymeric substances and microbial successions. Chemical Engineering Journal, 331, 841-849.
Huang, W., Li, B., Zhang, C., Zhang, Z., Lei, Z., Lu, B., et al. 2015. Effect of algae growth on aerobic granulation and nutrients removal from synthetic wastewater by using sequencing batch reactors. Bioresource Technology, 179, 187-192.
Jamshidi, M., Zinatizadeh, A., Rezaee, S. & Asadi, A. 2019. Process performance of a granular single bioreactor with continuous feeding and intermittent discharge regime treating dairy wastewater. International Journal of Engineering, 32, 10-17.
Jing, J., Feng, J., Li, W. & Xu, Y. 2009. Removal of COD from coking-plant wastewater in the moving-bed biofilm sequencing batch reactor. Korean Journal of Chemical Engineering, 26, 564-568.
Kim, D. S., Jung, N. S. & Park, Y. S. 2008. Characteristics of nitrogen and phosphorus removal in SBR and SBBR with different ammonium loading rates. Korean Journal of Chemical Engineering, 25, 793-800.
Lemaire, R., Yuan, Z., Bernet, N., Marcos, M., Yilmaz, G. & Keller, J. 2009. A sequencing batch reactor system for high-level biological nitrogen and phosphorus removal from abattoir wastewater. Biodegradation, 20, 339-350.
Liu, J., Yuan, Y., Li, B., Zhang, Q., Wu, L., Li, X., et al. 2017. Enhanced nitrogen and phosphorus removal from municipal wastewater in an anaerobic-aerobic-anoxic sequencing batch reactor with sludge fermentation products as carbon source. Bioresource Technology, 244, 1158-1165.
Mahardika, D. P. 2014. Aerobic Granulation with Industrial Wastewater in Sequencing Batch Reactors. PhD Thesis, Universiti Teknologi Malaysia.
Mansouri, A. M., Zinatizadeh, A. A., Irandoust, M. & Akhbari, A. 2014. Statistical analysis and optimization of simultaneous biological nutrients removal process in an intermittently aerated SBR. Korean Journal of Chemical Engineering, 31, 88-97.
Matinfar, A., Mohammadi, M., Najafpour, G. D. & Younesi, H. 2019. Ammonia and phosphorus removal from mixture of treated and raw cattle manure wastewater in a low‐O2 granular sequencing batch reactor. Journal of Chemical Technology and Biotechnology, 94, 2238-2247.
Montgomery, D. C. 2017. Design and Analysis of Experiments, John Wiley & Sons. Inc., New York.
Pan, M., Chen, T., Hu, Z. & Zhan, X. 2013. Assessment of nitrogen and phosphorus removal in an intermittently aerated sequencing batch reactor (IASBR) and a sequencing batch reactor (SBR). Water Science and Technology, 68, 400-405.
Pishgar, R. 2019. Nutrient (Ammonium and Phosphate) Removal Using Aerobic Granulation at Pilot scale. PhD Thesis, University of Calgary, Canada.
Rahimi, Y., Torabian, A., Mehrdadi, N. & Shahmoradi, B. 2011. Simultaneous nitrification–denitrification and phosphorus removal in a fixed bed sequencing batch reactor (FBSBR). Journal of Hazardous Materials, 185, 852-857.
Sánchez, A. S., Garrido, J. & Méndez, R. 2010. A comparative study of tertiary membrane filtration of industrial wastewater treated in a granular and a flocculent sludge SBR. Desalination, 250, 810-814.
Sharma, S., Sarma, S. J. & Tay, J. H. 2019. Aerobic granulation in wastewater treatment: a general overview. Microbial Wastewater Treatment. 2019, 57- 81. Elsevier.
Sheng, G. P., Yu, H. Q. & Li, X. Y. 2010. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnology Advances, 28, 882-894.
Su, K. Z. & Yu, H. Q. 2005. Formation and characterization of aerobic granules in a sequencing batch reactor treating soybean-processing wastewater. Environmental Science and Technology, 39, 2818-2827.
Wan, J., Bessière, Y. & Spérandio, M. 2009. Alternating anoxic feast/aerobic famine condition for improving granular sludge formation in sequencing batch airlift reactor at reduced aeration rate. Water Rresearch, 43(20), 5097-5108.
Wang, L. K., Hung, Y. T., Lo, H. H. & Yapijakis, C. 2004. Handbook of Industrial and Hazardous Wastes Treatment, CRC Press, New York, USA.
Wang, S. G., Liu, X. W., Gong, W. X., Gao, B. Y., Zhang, D. H. & Yu, H. Q. 2007. Aerobic granulation with brewery wastewater in a sequencing batch reactor. Bioresource Technology, 98, 2142-2147.
Wilén, B. M., Liébana, R., Persson, F., Modin, O. & Hermansson, M. 2018. The mechanisms of granulation of activated sludge in wastewater treatment, its optimization, and impact on effluent quality. Applied Microbiology and Biotechnology, 102, 5005-5020.
Yang, S., Yang, F., Fu, Z., Wang, T. & Lei, R. 2010. Simultaneous nitrogen and phosphorus removal by a novel sequencing batch moving bed membrane bioreactor for wastewater treatment. Journal of Hazardous Materials, 175, 551-557.
Yuan, Y., Liu, J., Ma, B., Liu, Y., Wang, B. & Peng, Y. 2016. Improving municipal wastewater nitrogen and phosphorous removal by feeding sludge fermentation products to sequencing batch reactor (SBR). Bioresource Technology, 222, 326-334.