Endotoxin Removal from Water Using Heterogenus Catalytic Ozonation by Bone Char

Document Type : Research Paper


1 Assoc. Prof. of Environmental Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran

2 Ph.D. of Environmental Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran

3 Assoc. Prof. of Environmental Health, Faculty of Medical Sciences, Shahid Beheshti University, Tehran

4 Prof. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran


The endotoxin is one of pollutants with lipopolysaccharide structure which release from gram negative bacteria and cyanobacters. The aim of this study was removal of endotoxin from water using catalytic ozonation by bone char. The endotoxin for experiments have extracted from Escherichia coli bacterium cell wall by Stefan and Jan method. Chromogenic limulus ambusite lysate method in 405-410 nm wave length was used for analysing of endotoxin. The ozone have analysed by potassium iodine method. Results: Results of the research shown endotoxin removal rates using heterogenous catalytic ozonation were 6.0 Eu/ml.min and 0.5 Eu/ml.min for grey bone char and white bone char, respectively. The efficency of the process was found eighty percent. Primary concentration of basic compounds had no effect on endotoxin removal rate. Therefore, endotoxin removal kinetic of reaction is a zero order reaction. This study revealed that ozonation process using bone char is more efficient than other proposed methods such as ozonation or chlorination and can be used successfully for endotoxin removal from water as a efficient method.


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