Reclamation of Phenol Contaminated Wastewater by Paenibacillus sp.

Document Type : Research Paper



Phenol and its compounds are toxic and hazardous material for environment even in low concentrations. So removal of these compounds from wastewaters is very important. There are many methods for removal of phenol from contaminated wastewaters among which biodegradation is more attractive. In this study presuming the availabity of phenol degrading-bacterium in phenolic wastewater, indigenous bacterium were isolated and identified by PCR. The growth curve and phenol removal of this bacterium were prepared. Results showed that the best isolated was the stain of Paenibacillus that can remove 1000 mg.l-1 phenol during 96 hours. The results showed that indigenous bacterium can remove high concentration of phenol from contaminated wastewater.


Main Subjects

1. Annadoria, G., Juang, R., and Lee, D. (2002). “Microbiological degradation of phenol using mixed liquors of Pseudomonas Putida and actived sludge.” Waste Management, 22, 703-710.
 2. Baker, M.D., and Mayfield, C.I. (1980). “Microbial and nonbiological decomposition of chlorophenols and phenol in soil.” Water, Air and Soil Pollution, 13, 411-424.
3. Chen, W., Chang, J., and Chang, S. (2004). “Characterization of phenol and trichloroethylene degradation by the rhizobium ralstonia taiwanensis.” J. Research in Microbiology, 155, 672-680.
4. Eskandary, S., Tahmourespour, A., and Hoodaji, M. (2011). “Investigation of growth and removal of phenol by isolation of bacterium from industrial waste water in vitro.” J. Water and Wastewater, 78, 78-85. (In Persian)
5. Freeman, H. (1989). Standard handbook of hazardous waste treatment and disposal, Mc Graw-Hill, USA.
6. Garrity, G., Brenner, J., and Krieg, R. (2001). Bergey s  manual of systematic bacteriology, 2nd Ed., Williams and Wilkins.
7. Golbang, M., Shahian, H. M., and Emtiazi, G. (2004). “Effect of phenol concentration on growth respiration and biofilm formation of phenol degradation bacteria in Esfahan steal plant wastewater.” J. Water and Wastewater, 61, 43-52. (In Persian)
8. Gonzalez, J., Herrera, G., Garcia, M.T., and Pena, M. (2001). “Biodegradation of phenol in a continuous process.” Bioresearches Technology, 76, 245-251.
9. Zhang, G., Ling, J., Sun, H. , Luo, J., Fan, Y., and Cuia, Z. (2009). “Isolation and characterization of a newly isolated polycyclic aromatic hydrocarbons-degrading Janibacter anophelis strain JY11.” J. of Hazardous Materials, 172, 580-586.
10. La, S., and Tabacchioni, S. (2009). “Ecology and biotechnological potential of Paenibacillus polymyxa: A mini review.” Indian J. Microbiol, 49, 2-10.
11. Gardener, M. (2004). “Ecology of Bacillus and Paenibacillus spp. in agricultural systems.” Phytopathology, 94, 1252-1258.
12. Neumann, G., Teras, R., Monson, L., Kivisaar, M., Schauer, F., and Heipieper, J. (2004). “Simultaneos degradation of atrazine and phenol by pseudomonas sp. Strain ADP: Effect of toxicity and adaptation.” Applied and Environmental Microbiology, 70, 1907-1912.
13. Ouyang, J., Pei, Z., Lutwick, L., Dalal, S., Yang, L., Cassai, N., Sandhu, K., Hanna, B., Wieczorek, R.L., Bluth, M., and Pincus, M.R. (2008). “Paenibacillus thiaminolyticus: a new cause of human infection, inducing bacteremia in a patient on hemodialysis.” J. Ann. Clin. Lab. Sci., 38, 393-400.
14. Patterson, J.W. (1975). Wastewater treatment technology, Ann. Arbor Science Publishers, Inc.,USA.
15. Pession, E., Divari, S., Griva, E., Cavaletto, M., Rossi, G.L., and Giunta, C. (2000). “Phenol hydroxylase from acinetobacter radioresistensis a multi component enzyme.” Biochemistry Journal, 265, 162-171.
16. Rehm, H., and Reed, G. (1999). Biotechnology, 2nd Ed., Vol. 11a. WIVY-VCH, Weinbeim Germany. 
17. Environmental Protection Agency. (2004). Collation of toxicological data and intake values for humans, EPA report, USA.
18. Termamoto, M., Ohnishi, K., Harayama, S., and Watanabe, K. (2002). “An rac/xy1s family member at a high level in a hierarchy of regulators for phenol metabolizing enzymesin comamonas testosterone:R5.” Applied and Environmental Microbiology Journal, 184, 3941-3946.
19. Shih, C., Davey, M., Zhou, J., Tiedje, J., and Criddle, C. (1996). “Effect of phenol feeding pattern on microbial community structure and co metabolism of trichloroethylene.” Applied and Environmental Microbiology, 62, 2953-2960.
20. Rigo, M., and Alegre, R.M. (2004). “Isolation and selection of phenol-degrading microorganisms kinetics of the biodegradation.” Folia Microbiology, 49, 41-45.
21. Robert, J., Shimpand, F., and Pfaender, K. (1987). “Effect of adaptation to phenol on biodegradation of Mono Substituted Phenol Aquatic Microbial Communities.” Applied and Environmental Microbiology, 53, 1496-1499.
22. Chandra, R., Yadva, R. Bharagava, R. and Rai, V. (2011). “Phenol degradation by Paenibacillus thiaminolyticus and Bacillus cereus in axenic and mixed conditions.” World Journal of Microbiology and Biotechnology, 27, 2939-2947.
23. Yang, C., and Lee, C.M. (2006). “Enrichment, isolation and characterization of phenol-degrading pseudomonas resinovornas strain P-1 and brevibacillus sp. Strain P-6.” International Biodetrioration and Biodegradation Journal, 59, 206-210.
24. Watanabe, K., Teramoto,  M., Futamata, H., and Harayama, S. (1998). “Molecular detection , isolation , and physiological characterization of functionally dominant phenol – degrading bacteria in activated sludge.” Applied and Environmental Microbiology, 64, 4399-4402.
25. Whiteley, A. S., and Bailey, M. J. (2000). “Bacterial community structure and physiological state within an industrial phenol bioremediation system.” J. Applied and Environmental Microbiology, 66, 2400-2407.
26. Zhang, Y., Lu, D. Ju, T., Wang, L., Lin, Sh., Zhao, Y., Wang, Ch., He, H., and Du, Y. (2013). “ Biodegradation of phenol using bacillus cereus WJ1 and evaluation of degradation efficiency based on a graphene-modified electrode.” Int. J. Electrochem. Sci., 8, 504-519.
27. Naresh, B., Honey, P., and Vaishali, S. (2012). “Biodegradation of phenol by a bacterial strain isolated from a phenol contaminated site in India.” Research Journal of Environment Sciences, 1, 46-49.