A Simple and Rapid Method to Evaluate Potentially Mineralizable Nitrogen in Sewage Sludge Amended Calcareous Soils

Document Type : Research Paper


1 Former Graduate Student, of Soil Science, Isfahan University of Technology

2 Assistant Professor of Soil Science, Isfahan University of Technology

3 Associate Professor of Soil Science, Isfahan University of Technology


Potentially mineralizable nitrogen (PMN) can be usually considered as labile nitrogen. Measurement of PMN is expensive and time consuming; therefore, a simpler and more rapid alternative may facilitate routine laboratory analysis. The objective of this study was to determine the relationship between PMN and biological index of nitrogen availability (BINA). The studied soil was previously treated with 0, 25, and 100 tons ha-1 of sewage sludge with 0, 1, 2 and 3 consecutive years of application. Soil samples were taken 6 months after the latest application. PMN was measured according to Stanford and Smith procedure (20 weeks of aerobic incubation with 2 weeks leaching intervals) and BINA measured as described by Bundy and Meisinger (7 days of anaerobic incubation at 40˚ C followed by extraction of NH4+). Results showed that PMN was significantly correlated with BINA (r = 0.938, P<0.001) in sewage sludge treated plots and that BINA can be considered as a more reliable index for estimation of PMN. The results showed that BINA might be used as a simple and rapid method to evaluate PMN in sludge amended calcareous soils.


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شماره 54- سال 1384                                                                                                                        آب و فاضلاب
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