Optimizing Biodegradation of Floating Diesel Fuel Contaminated Wastewater Using the Taguchy Method

Document Type : Research Paper


1 M.Sc. and faculty member of Chemical Engineering Civil and Environmental Engineering Dept., Jami Institute of Technology, Delijan

2 Assis. Prof. of Chemical Engineering, Faculty of Chemical Engineering, University of Isfahan

3 Assoc. Prof. of Environmental Health, Faculty of Public Health, Ahwaz University of Medical Sciences


In this study, biodegradation of floating diesel fuel was investigated by using two gram negative strains designated as A1 and A2 which were isolated from a reservoir tank of a gas station in Isfahan. One of them was identified as pseudomonas aeruginosa. The percent removal of diesel fuel was evaluated for pure and mixture cultivations. The highest removal percent belomged to A1, which was able to remove 88% of the floating diesel fuel. Also the evaluation of emulsification index (E24) of the cultivation mixture indicates that the microorganisms can produce a significant amount of biosurfactant. The emulsification index values for A1 and A2 were 23% and 21%, respectively. pH, nitrogen source concentration, fuel concentration, and salinity at three levels were investigated and the optimum values of these parameters were determined at 8, 0.55 g/lit, 2%, and 8%, respectively.


1- Tellez, G. T., Nirmalakhandan, N., and Gardea-Torresdey, J., L. (2002). “Performance evaluation of an activated sludge system for removing petroleum hydrocarbons from oilfield produced water.” Advances in Environmental Research, 6 (4), 455-470.
2- Talaie, A.R. (2008). “Parametric study of petroleum compounds biodegradation using microorganisms.” M.Sc. Thesis of Environmental Engineering, Scince andResearchUniversity Branch-Ahvaz.
3- Capelle, F.H. (1993). Ground-water microbiology and geochemistry, John Wiley and Sons, Inc, N.Y.
4- Neff, J. M., Sauer, T.C., Macialek, N. (1992). “Composition, fate and effects of produ cedwater discharges to nearshore manne waters.” Environ. Sci. Res., 46, 371-385.
5- Hua, J. (2006). “Biodegradation of dispersed marine fuel oil in sediment under engineered pre-spill application strategy.” Ocean Engineering, 33 (2), 152-167.
6-Vieira, P.A., Vieira, R. B., Franc, F.P., and Cardoso, V.L. (2007). “Biodegradation of effluent contaminated with diesel fuel and gasoline.” Journal of Hazardous Materials, 140 (1-2), 52-59.
7- Tiburtius, E. R. L., Zamora, P. P., and Leal, E.S. (2004). “Contamination of waters by BTXs and processes used in the remediation of contaminated sites.” Qu´ım. Nova., 27, (3), 441-446.
8- Gogoi, B.K., Dutta, N. N., Goswami, P. and KrishnaMohan, T.R. (2003). “A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site.” Adv. Environ. Res., 7, 767-782.
9- Townsend, G. T., Prince, R. C., and Suflita, J. M. (2004). “Anaerobic biodegradation of alicyclic constituents of gasoline and natural gas condensate by bacteria from an anoxic aquifer.” FEMS Microbiol. Ecol, 49 (1),
10- Kaluarachchi, J. J., Cvetkovic, V., and Berglund, S. (2000). “Stochastic analysis of oxygen- and nitrate-based biodegradation of hydrocarbons in aquifers.” J. Cont. Hydrol., 41, 335-365.
11- Bielicka, K., Kaczorek, E., Olszanowski, A., and Voelkel, A. (2002). “Examination of biodegradation of hydrocarbons in emulsified systems.” Poblish J. Environ. Stud., 11 (1), 11-16.
12- Diaz, M. P., Grigson, S. J. W., Peppiatt, C.J., and Burgess, J.G. (2000). “Isolation and characterization of novel hydrocarbon-degrading euryhaline consortia from crude oil and mangrove sediments.” Mar. Biotech.,
2 (6), 522-532.
13- Lakha, S. S., Miller, M., Campbell, R.G., Elahimanesh, K. S. P., Hart, M.M., and Trevors, J.T. (2005). “Microbial gene expression in soil: methods, applications and challenges.”J. Microbiol. Method, 63 (1), 1-19.
14- Grishchenkov, V.G. Townsend, R.T. Mcdonald, T.J. Autenrieth, R.L. Bonner, J.S., and Boronin, A.M. (2000). “Degradation of petroleum hydrocarbons by facultative anaerobic bacteria under aerobic and anaerobic conditions.”Process Biochem., 35 (9), 889-896.
15- Li, Q., Congbao, K., and Changkai Z. (2005). “Wastewater Produced from an oilfield and Continuous Treatment with and Oil-Degrading Bacterium.” Process Biochemistry, 40 (2), 873-877.
16- Dibble, J.T., and Bartha, R. (1979). “Effect of environmental parameters on the biodegradation of oil sludge.” Applied and Environmental Microbiology, 37 (4) 729-739.
17- Liang, Z. G., Wu Y. T., Qian X. P., and Meng, Q. (2005). “Biodegradation of crude by Pseudomonas Aeruginosa in the presence of rhamnolipids,” Journal of Zhejiang University Science, 6B(8), 725-730.
18- Meintanis, C., Kalliopi, I.C., Konstantinos- Kormas, A., and Amalia D. K., (2006). “Biodegradation of crude oil by thermophilic bacteria isolated from a VolcanoIsland.” Biodegradation,17(2), 3-9.
19- Kabiri, K. (2008). “Biodegradation of petroleum pollutant from soil.” M.Sc Thesis of Biotechnology,EsfehanUniversity.
20- Daneshvar, N., Khataee, A.R., Rasoulifard, M., and Pourhassan, M. (2007). “Biodegradation of dye solution containing Malachite Green: Optimization of effective parameters using Taguchi method.” Journal of Hazardous Materials, 143 (1-2), 214-219.
21- Urum, k., Pekdemir, T., and Copur M. (2004). “Surfactants treatment of crude oil contaminated soils.” Journal of Colloid and Interface Science, 276 (2), 456-464.
22- Tchobanoglous, G., and Burton, F. (2003). Wastewater engineering treatment and reuse, 4thEd., McGraw Hill, Metcalf and Eddy Inc.,New York.