Heavy Metals and Antibiotic Co-Resistance in Bacterial Isolates of Industrial Effluents

Document Type : Research Paper


Assoc. Prof., Dept. of Basic Medical Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran


Heavy metal and antibiotic co-resistance is a global issue. The goal of this research was to explore the heavy metal, also antibiotic resistance patterns of effluent bacterial isolates. Heavy metal resistant bacteria were isolated from effluents and their Minimum Inhibitory Concentration (MIC) was determined. The Multi-Metal resistance (MMR) pattern and antibiotic resistance trait of isolates were defined. The MIC of Cu2+, Pb2+, Cd2+ and Zn2+ was 4, 8, 12 and 24 mM/L, respectively. Most of the isolates indicated the Cd2+, Pb2+ and Zn2+ resistance and high resistance to the most tested antibiotics. The 16S rDNA gene sequences of resistant isolates were handed over to NCBI-GenBank as Staphylococcus sp. ATHA2(JX120151) and Klebsiella oxytoca ATHA1(JQ928574). Correlation was found between metal tolerances, heavy metal concentration, also antibiotic resistance in bacteria. Thus, it is important to not only be aware of antibiotics misapplication, but also respond to excessive discharge of effluent containing heavy metals to the environment.


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