Mechanism of Biosorption of Nickel Ions from Polluted Effluent by Bacillus sp. Strain MGL-75

Document Type : Research Paper

Authors

Abstract

The aim of this work was to investigate Bacillus sp. strain MGL-75 as biosorbent, for the fixation of Ni ion in batch reactor. Pollution of the environment by toxic metals is a major environmental concern. In a first step, biosorption kinetics and isotherms have been performed at pH 7. The equilibrium time was about 5 min and the adsorption equilibrium data were well described by the Langmuir`s equation. The point of zero net proton charge (PZNPC) was found close to pH 5.7. Using the single extrapolation method, three kinds of acidic functional groups with three intrinsic pka were determined at 4.4, 6.9 and 11.2. The maximum capacity has been extrapolated to 0/52 mmol/g. Finally the effect of autoclave, 2, 4 Dinitrophenol (DNF) and Na-Azid (NaN3), and the effect of pH values, were studied. These results indicated that the Bacillus sp. strain MGL-75 is an excellent candidate for use in reactor to remove Nickel ions from polluted aqueous effluents.

Keywords


1- Marandi, R., Hojatti, A., and Akhavan Sepehi, A. (2009). “Biosorption of Cr (II) onto biomass of Aspergillus niger.” Environmental Science and Technology, 12, 23-25.
2- Salkhori, N.Y., Ghaemi, N., and Nohi, A. (2010). “Biosorption of heavy metal onto microorganism.” J. of Biological Science, 4, 51-60.
3- Gadd, G.M., and White, Ch. (1993). “Microbial treatment of metal pollution a working biotechnology.” Elsevier Science Publishers LTD, 11, 353-359.
4- Volesky, B., and May-Phillips, H.A. (1995). “Biosorption of heavy metal by Saccharomyces cervisiae.” Applied Microbiology Biotechnology, 40, 783-797.
5- Reddad, Z., Gerente, C., Andres, Y., and Le Cloirec, P. (2002). “Adsorption of several metal ions onto a low cost biosorbent: Kinetic and equilibrium studies.” Environmental Science Technology, 36, 2067-2073.
6- Sabry, S.A., Ghozlan, H.A., and Abouzeid, M. (1997). “Metal tolerance and antibiotic resistance patterns of a bacterial population isolated from sea water.” J. of Applied Microbiology, 82, 245-252.
7- Leusch, A., Holan, Z. R., and Volesky, B. (1995). “Biosorption of heavy metal (Cd, Cu, Ni, Pb, Zn) by chemically – reinforced bioman of marine algae.” J. of Chemical Technology and Biotechnology, 62, 279-283.
8- Ahmady-Asbchin, S., Andres, Y., Gérente, C., and Le Cloirec, P. (2008). “Biosorption of Cu (II) from aqueous solution by Fucus serratus.” Bioresource Technology, 99, 6150-6155.
9- Macaskie, L.E. (1990). “An immobilization cell bioprocess for the removal o heavy metals from aqueous flows.” J. of Chemical Technology and Biotechnology, 49, 357-379.
10- Langmuir, I. (1916). “The constitution and fundamental properties of solids and liquids.” J. of the American Chemical Society, 38, 2221-2295.
11- Hafez, M., Abdel-Razek, A.S., and Hafez, M.B. (1997). “Accumulation of heavy metals on Aspergillus flavus.” J. of Chemical Technology and Biotechnology, 68, 1001-1003.
12- Pumpel, T., Pernfub, B., Pigher, B., Diels, L., and Schinner, F. (1995). “A rapid screening method for the isolation of metal, accumulating microorganisms.” J. of Industrial Microbiology, 14, 213-217.
13- Stumm, W., and Morgan, J.J. (1996). In aquatic chemistry, chemical equilibria and rates in natural waters, 3th Ed., John Wiley and Sons, NY.
14- Rom, D.L., and Gadd, G.M. (1991). “Use of pelleted and immobilized yeast and fungal biomass for heavy metal and radionuclide recovery.” J. of Industrial Microbiology, 7, 97-104.
15- Ping Xin, S., Ting, Y., Chen, J. Pl., and Hong, L. (2004). “Sorption of lead, copper, cadmium, zinc and nickel by marine algal biomass: Characterization of biosorptive capacity and investigation of mechanisms.” J. of Colloid and Interface Science, 275, 131-141.
16- Holan, Z.R., and Volesky, B. (1994). “Biosorption of lead and nickel by biomass of marine algae.” Biotechnology and Bioengineering, 43, 1001- 1009.
17- Prasher, S.O., Beaugeard, M., Hawari, J., Bera, P., Patel, R.M., and Kim, S.H. (2004). “Biosorption of heavy metal by red algae (Palmaria palmata).” Environmental Technology, 25, 1097-1106.
18- Ahmady-Asbchin, S., Andres, Y., Gerente, C., and Le Cloirec, P. (2009). “Natural seaweed waste as sorbent for heavy metal removal from solution.” Environmental Technology, 30, 755-762.
19- Pardo, R., Herguedas, M., Barrado, E., and Vega, M. (2003). “Biosorption of cadmium, copper, lead and zinc by inactive biomass of Pseudomonas putida.” Anal Bioanal Chem., 376, 26-32.
20- Masud Hossain, S.K., and Anantharaman, N. (2006). “Studies on bacterial growth and lead (II) biosorption using Bacillus subtilis.”Indian J. of Chemical Technology, 13, 591-596.