Journal of Water and Wastewater; Ab va Fazilab (in persian)

Journal of Water and Wastewater; Ab va Fazilab (in persian)

Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor

Document Type : Research Paper

Authors
Soroush Dayani Najafabadi 1
Maryam Hasani Zonoozi 2
Roya Aliaskarian 3
1 Master of Environmental Engineering, Dept. of Civil Engineering, Iran University of Science and Technology (IUST), Tehran, Iran
2 Assist. Prof., Water and Environmental Engineering Group, Dept. of Civil Engineering, Iran University of Science and Technology (IUST), Tehran, Iran
3 Master of Microbiology, Dept. of Molecular Cell Biology and Microbiology, Isfahan University, Isfahan, Iran
10.22093/wwj.2024.474675.3432
Abstract
Biosustainability, toxicity, and the potential for disruption of aquatic ecosystems and human health have made azo dyes into a serious environmental issue in industrial wastewater. In this context, a group of bacteria isolated from dye-contaminated wastewater was used in a moving bed bioreactor with polyurethane foam biocarriers for the biodegradation of Congo red azo dye. According to the EzBioCloud database (eztaxon), the dominant bacterium identified through DNA extraction, PCR, and 16S-rRNA sequencing showed the highest similarity to Lysinibacillus Capsici PB300(T). The efficiency of dye and chemical oxygen demand (COD) removal in the MBBR was examined in relation to process time, filling ratio, and dye concentration. Dye removal in this system was optimized, maintaining the pH at 7 ± 0.2, the temperature at 35 ± 1.0 °C, and COD at 980 mg/L. The highest removal efficiencies for dye and COD were 92% and 85.1%, respectively, after 72 hours at a dye concentration of 50 mg/L and a filling ratio of 40%. Kinetic modeling was employed to study biodegradation. The R² value for the first-order model was 0.9457, while for the second-order model it was 0.9791. The second-order kinetic model (Grau's equation) provided a more accurate prediction of dye degradation in the MBBR system. This study confirms the successful degradation of Congo red azo dye using immobilized bacteria on Polyurethane foam (PUF) biocarriers in the MBBR bioreactor.
Keywords
Congo Red Azo Dye
Polyurethane Foam
Moving Bed Bioreactor
Lysinibacillus Capsici PB300(T)
Biodegradation
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Journal of Water and Wastewater; Ab va Fazilab (in persian)
Volume 35, Issue 3 - Serial Number 152
September and October 2024
Pages 45-68