Journal of Water and Wastewater; Ab va Fazilab (in persian)

Journal of Water and Wastewater; Ab va Fazilab (in persian)

Isolation of Antibiotic-Resistant Enterobacteriaceae from Poultry Wastewater

Document Type : Research Paper

Authors
Armaghan Nikoosokhan 1
Azam Haddadi 2
Naser Harzandi 2
1 Master of Microbiology, Dept. of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2 Assist. Prof., Dept. of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
10.22093/wwj.2024.440391.3398
Abstract
Antibiotic-resistant bacteria have increased in livestock and poultry over recent years. However, to date, there have been few studies that have investigated the antibiotic resistance bacteria among environmental and animal samples. Hence, the main purpose of this research was to isolate Enterobacteriaceae from the wastewater of poultry farms and investigate the pattern of antibiotic resistance in these bacteria. In this study, 100 wastewater samples were isolated from different poultry farms. These strains were identified using biochemical tests. The antibiotic resistance pattern was determined by the disk diffusion method (Kirby–Bauer). The isolated bacteria were investigated in terms of resistance patterns to cephalexin, tylosin, doxycycline, and erythromycin. A total of 170 Gram-negative bacteria from the Enterobacteriaceae family were isolated from poultry wastewater samples. The most isolated strain was Escherichia coli (35.33%), followed by Klebsiella (17.65%). According to this study, the most resistance was to cephalexin and doxycycline antibiotics. The results of this research indicate a high level of antibiotic resistance in bacterial isolates from wastewater. This is probably be due to the improper use of antibiotics in the poultry industry.
Keywords
Antibiotic Resistance
Antibiotic
Enterobacteriaceae
Poultry Industry
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Journal of Water and Wastewater; Ab va Fazilab (in persian)
Volume 35, Issue 1 - Serial Number 150
March and April 2024
Pages 76-85