بررسی روند رشد و تجزیه زیستی فنل به‌وسیله باکتری جدا شده از پساب صنعتی در شرایط آزمایشگاه

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای خاکشناسی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی، واحد خوراسگان، اصفهان

2 دانشیار گروه خاکشناسی، دانشگاه آزاد اسلامی، واحد خوراسگان، اصفهان

3 استادیار گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد خوراسگان،اصفهان

چکیده

فنل یک ترکیب کربن‌دار است که در مقادیر بیشتر از 0/5 میلی‌گرم در لیتر برای محیط و انسان خطرناک است. بهترین روش برای تصفیه و از بین بردن فنل موجود در پساب بخش کک‌سازی کارخانه‌های ذوب فلز، تصفیه زیستی است. در این تحقیق با جداسازی باکتری‌های بومی موجود در پساب فنل‌دار کارخانه ذوب آهن اصفهان، اقدام به سازش‌پذیر کردن یک جدایه و در نهایت حذف فنل توسط این جدایه گردید. همچنین رفتار این جدایه در محیط کشت سنتزی حاوی 2000 و 4000 میلی‌گرم در لیتر فنل بررسی شد و مشخص شد که این جدایه پس از یک فاز تأخیری 24 و 48 ساعته رشد کرده و مقدار فنل را به‌ترتیب پس از 264 و 288 ساعت به صفر میلی‌گرم در لیتر می‌رساند. این جدایه قادر است مقدار فنل را در یک پساب طبیعی از 2233 میلی‌گرم در لیتر در طی مدت 120 ساعت به صفر برساند. شناسایی این جدایه مشخص کرد یک کوکوباسیل گرم منفی است که احتمالاً از گونه سدوموناس است. با به‌کارگیری این جدایه به‌تنهایی و یا حتی ترکیبی از چند جدایه سازگار شده، می‌توان میزان فنل در این پساب‌ها را در طی مدت زمان کوتاه‌تری به صفر رساند.
 

کلیدواژه‌ها


عنوان مقاله [English]

Study of Growth Process and Phenol Biodegradation by a Bacterium Isolated from Wastewater (in vitro)

نویسندگان [English]

  • Somayye Eskandary 1
  • Mehran Hoodaji 2
  • Arezoo Tahmourespour 3
1 Ph.D. Student of Soil Sciences, School of Agriculture and Natural Resources, Azad University, Khorasgan Branch, Isfahan
2 Assoc. Prof. of Soil Science, Islamic Azad University, Khorasgan Branch, Isfahan
3 Assist. Prof. of Microbiology, Faculty of Sciences, Islamic Azad University, Khorasgan Branch, Isfahan
چکیده [English]

Phenol is a carbonic compound that is dangerous for humans at a concentration of 0.5 ppm in the environment. The best phenol removal from coal tar wastewaters is achieved by bioremediation. In this study, we isolated indigenous bacteria from phenolic wastewater and adapted it to a high concentration of phenol for its removal from wastewater. We also investigated the growth and removal curves of the bacteria in media with 2000 and 4000 ppm of phenol. It was observed that after lag phases of 24 and 48 hours, they grew and removed all of the phenol concentration over 264 and 312 hours. It was also found that this isolate was able to remove 2233 ppm of phenol in natural wastewater over a period of 120 hours. Identification tests showed that it is a gram-negative bactetium possibly belonging to the pseudomonas species. Phenol concentrations in wastewater can be reduced over a shorter period of time by using either this isolate alone or a group of them.
 

کلیدواژه‌ها [English]

  • Biodegradation
  • Phenol
  • isolation
  • Bacteria
  • Wastewater
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