جذب زیستی یون سرب توسط سودوموناس جدا شده از پساب‌های آلوده نفتی خوزستان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار میکروبیولوژی، گروه زیست شناسی، دانشگاه آزاد اسلامی، تنکابن

2 کارشناس ارشد میکروبیولوژی، گروه زیست شناسی، دانشگاه آزاد اسلامی، تنکابن

چکیده

جذبزیستییکیازفناوری‌هایمؤثردرحذففلزاتسنگین است. هدف از این پژوهش، جداسازی سودوموناس مقاوم و تعیین شرایط بهینه رشد آن، تعیین حداقل غلظت بازدارنده و بررسی حذف زیستی سویه بود. برای انجام پژوهش، 5 نمونه از پساب‌های نفتی مناطق خوزستان در شرایط سترون جمع‌آوری شد و به آزمایشگاه منتقل شد. نمونه‌های همگن رقیق‌شده پساب، بر روی محیط لوریا برتانی آگار دارای ppm 5 لید نیترات کشت داده شدند و پس از گذشت 24 ساعت در دمای 37 درجه سلسیوس، پرگنه سویه‌های مقاوم برای غربالگری روی محیط مکانکی آگار کشت داده شد. این باکتری‌ها با آزمون‌های بیوشیمیایی شناسایی شدند. در مجموع 24 سویه سودوموناس جدا شد که 10 سویه مقاوم به سرب بود. حداقل غلظت مهارکنندگی رشد، برای غربالگری و جداسازی سویه‌های مقاوم از 100 تا ppm 2100 انجام شد. سپس پلیت‌ها در دمای 37 درجه به‌مدت 24 ساعت نگهداری شدند و در این بین، سویه Mso1 برای آزمایش‌های فراتر انتخاب شد. آزمایش تعیین حساسیت آنتی‌بیوتیکی نشان داد که سویه مربوطه نسبت به کلرامفنیکل به‌میزان 30 میکروگرم و اریترومایسین به‌میزان 15 میکروگرم مقاوم است. رشد بهینه باکتری در حضور سرب در دمای 40 درجه سلسیوس، سرعت تکان rpm 100 و pH برابر با 6، با روش اسپکتروفتومتری در600 نانومتر تعیین شد. در این مطالعه سویه Mso1ظرف مدت 24 ساعت، 45/38 درصد سرب را از محیط حذف کرد. این پژوهش، اهمیت استفاده از گونه‌های سودوموناس را در اصلاح زیستی پساب آلوده به سرب آشکار می‌کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Biosorption of Lead by Pseudomonas sp Isolated from Oil-Contaminated Wastewaters in Khuzestan

نویسندگان [English]

  • Seyed Mansour Meybodi 1
  • Homa Khorasani 2
1 Assist. Prof. of Microbiology, Dept. of Biology, Islamic Azad University, Tonekabon
2 . M.Sc. in Microbiology, Dept. of Biology, Islamic Azad University, Tonekabon
چکیده [English]

Biosorption is a most effective technology for the removal of such toxic substances as heavy metals. The objective of this study was four-fold: 1) to isolate lead resistant Pseudomonas strains, 2) to determine the optimal conditions of their growth, 3) to obtain the minimum inhibitory concentration of lead, and 4) to evaluate the bioremoval of lead from culture solutions. For the purposes of this study, oil-contaminated wastewater samples were collected from Khuzestan region and transferred to laboratory where they were homogenized and serially diluted up to 10-10 with sterile saline before they were cultured in Luria Bertani agar medium containing 5ppm of lead nitrate. Resistant strains were then isolated at 37°C for 24h. The samples were subsequently cultured in Macconkey agar for isolation of appropriate gram negative strains. Biochemical tests were used to identify the bacteria, 10 strains of which were screened as lead resistant ones from all the 24 isolates. The bacterial colonies were selected and tested with different concentrations (100- 2100 ppm) of lead for their resistance. The plates were then incubated at 37ºC for 24h and Mso1 was chosen from among the lead resistant colonies for further experiments. This strain showed resistance to chloramphenicol (30µg) and erythromycin (15µg) when subjected to the antimicrobial susceptibility test. Optimal growth conditions included a temperature of 40°C at 100 rpm and a pH level of 6 in the presence of lead by spectrophotometry at 600nm. Absorption tests showed that the Mso1 strain had a metal removal efficiency of 38.45% from an aqueous solution containing 100 ppm of lead over 24h. The results confirmed the capability of Pseudomonassp in the bioremediation of Pb-contaminated wastewaters.

کلیدواژه‌ها [English]

  • Biosorption
  • Lead
  • Pseudomonas
  • Oil-contaminated Wastewater
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