ساخت آزمایشگاهی حسگر میکربی اندازه‌گیری BOD نمونه‌های پساب صنعتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد مهندسی شیمی، دانشکده مهندسی شیمی، دانشگاه صنعتی اصفهان

2 استادیار بیوتکنولوژی محیط زیست، دانشکده مهندسی شیمی، دانشگاه صنعتی اصفهان

3 دانشیار، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان

چکیده

 در این تحقیق یک حسگر میکربی برای اندازه گیری BOD طراحی شد. در ساختار این حسگر از یک سلول کلارک به‌عنوان ترانسفورماتور و از لجن فعال تهیه شده از تصفیه‌خانه آب و فاضلاب شاهین شهر اصفهان به‌عنوان شناساگر حسگر استفاده شد. نتایج مربوط به کالیبراسیون حسگر نشان می‌دهد رابطه خطی بین اختلاف جریان الکتریکی و غلظت محلول استاندارد گلوکز- گلوتامیک اسید، تا مقدار BOD5 برابر با 50 میلی‌گرم اکسیژن در لیتر برقرار است. مقدار BOD فاضلابهای ورودی و پسابهای خروجی از واحدهای تصفیه‌خانه شرکت مواد غذایی آردینه، شاهین‌شهر اصفهان و شرکت پگاه اصفهان توسط حسگر اندازه‌‌گیری شد. مقایسه نتایج به‌دست آمده از حسگر و نتایج حاصل از روش استاندارد BOD5 نشان داد میانگین درصد خطای اندازه‌گیری BOD نمونه‌های ذکر شده توسط حسگر، برابر با 6/29 درصد است و نتایج مربوط به ارزیابی پایداری حسگر طراحی شده نشان داد مدت زمان پایداری پاسخ حسگر برابر با 3 روز است.

کلیدواژه‌ها


عنوان مقاله [English]

Construction of Microbial-based Biosensor to Measure BOD of Industrial Wastewaters

نویسندگان [English]

  • Behnam Mahdavi 1
  • Hamid Zilouei 2
  • Nourollah Mirghaffari 3
چکیده [English]

In this study a cell-based biosensor for measurement of BOD was designed and developed. Activated sludge collected from wastewater treatment plant of Shahinshahr was used as biological receptor and a Clark cell was used as transducer. According to the results obtained from the sensor calibration, a linear relationship between the current changes and glucose-glutamic acid (GAA) standard concentrations up to 50 mg/L was observed. The BOD values of different industrial wastewaters, inlet and outlet of treatment plant of Ardineh Company (Isfahan), and also  inlet and outlet of domestic wastewater treatment plant of Shahinshahr, and outlet of treatment plant of Pegah Company (Isfahan) were measured using this biosensor. Comparison of the results of this biosensor and the results of the standard BOD test (BOD5) showed that the mean percentage error measured by the sensor was +29.6%. The results concerning the stability of the designed biosensor showed a stability time of 3 days for the response of biosensor.

کلیدواژه‌ها [English]

  • Microbial-based Biosensor
  • Industrial Wastewater
  • Biochemical Oxygen Demand (BOD)
  • Activated sludge
  • Flow injection analysis
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