حذف زیستی فنل به‌کمک باکتری بومی جداسازی شده از خاک منطقه ذوب آهن اصفهان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای خاکشناسی، دانشگاه آزاد اسلامی، واحد خوراسگان، اصفهان

2 دانشیار گروه خاکشناسی، دانشگاه آزاد اسلامی، واحد خوراسگان، اصفهان

3 استادیار میکرب شناسی گروه علوم پایه پزشکی، دانشگاه آزاد اسلامی واحد خوراسگان، اصفهان

چکیده

فرایندهای صنعتی از جمله فعالیتهای بشر هستند که سبب تولید حجم بالایی از پساب حاوی آلاینده‌های آلی از جمله فنل و مشتقات آن می‌شوند. این آلاینده‌ها از جمله موادی هستند که برای سلامت محیط زیست، خاک و انسان تهدید جدی به‌حساب می‌آیند. برای پاکسازی منابع خاکی آلوده به فنل روشهای زیادی وجود دارد که از جمله این روشها حذف زیستی آن است. این تحقیق با هدف حذف زیستی فنل به جداسازی باکتری های تجزیه کننده از خاک آلوده به فنل در مجاورت استخر ایزوله کارخانه ذوب آهن اقدام شد. پس از نمونه برداری اسیدیته، مقدار مواد آلی، هدایت الکتریکی و غلظت فنل با هدف امکان وجود شرایط مساعد برای رشد باکتری تعیین گردید. همچنین اقدام به غنی‌سازی، جداسازی، شناسایی و بررسی روند تجزیه فنل توسط جدایه برتر گردید. نتایج نشان داد بهترین گونه در خاک مورد مطالعه، گونه‌ای از انتروباکتر است که توانایی حذف 400 میلی‌گرم در لیتر فنل را در طی مدت زمان 72 ساعت دارد. نتایج این تحقیق می‌تواند تأکیدی بر استفاده باکتری‌ها برای تصفیه آلاینده‌های آلی مانند فنل از محیط خاک باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Biodegradation of Phenol by Indigenous Bacterium Isolated from Contaminated Soil of Esfahan Steel Company Zone

نویسندگان [English]

  • Somayeh Eskandary 1
  • Mehran hoodaji 2
  • Arezoo Tahmorespour 3
چکیده [English]

Industrial processes produce high volumes of wastewater containing organic pollutants such as phenol and its derivatives. These contaminants include materials that are serious threats for environmental, human and soils characteristics . There are lots of methods for phenol removing from contaminated soils that one of them is biodegradation. In this study with the aim of phenol biodegradation, take action to isolation of bacteria from contaminated soil of Esfahan steel factory. Some properties of soil such as pH, organic matters, electrical conductivity and phenol concentration were determined. Enrichment of isolated bacteria was done. The best indigenous bacterium was selected according to its growth in presence of 400 mg.L-1 of phenol during 24h for further biodegradation studies and molecular identification. Results showed that according to phylogenetic analysis, the best isolated bacterium was the strain of Enterobactersp. that could remove 400 mg.L-1phenol during 72 hours. The results of this study shown that indigenous bacterium can remove organic pollutant such as phenol from contaminated soil.

کلیدواژه‌ها [English]

  • Contaminated Soil
  • Steel Company
  • Phenol
  • Indigenous Bacterium
  • Biodegradation
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